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phosphorylated yap1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated yap1
    Representative western blot and quantitative data of <t>p-YAP1,</t> YAP1, p-AKT, AKT, p-GSK3β and GSK3β protein expression (n = 4 per group). * P < 0.05 vs. CTL + AAV9-GFP group. # P < 0.05 vs. TAC + AAV9-GFP group.
    Phosphorylated Yap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated yap1/product/Cell Signaling Technology Inc
    Average 98 stars, based on 775 article reviews
    phosphorylated yap1 - by Bioz Stars, 2026-02
    98/100 stars

    Images

    1) Product Images from "Upregulated LIMD1 alleviates pressure overload-induced cardiac hypertrophy via inhibits YAP1/AKT/GSK3β signaling"

    Article Title: Upregulated LIMD1 alleviates pressure overload-induced cardiac hypertrophy via inhibits YAP1/AKT/GSK3β signaling

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0316149

    Representative western blot and quantitative data of p-YAP1, YAP1, p-AKT, AKT, p-GSK3β and GSK3β protein expression (n = 4 per group). * P < 0.05 vs. CTL + AAV9-GFP group. # P < 0.05 vs. TAC + AAV9-GFP group.
    Figure Legend Snippet: Representative western blot and quantitative data of p-YAP1, YAP1, p-AKT, AKT, p-GSK3β and GSK3β protein expression (n = 4 per group). * P < 0.05 vs. CTL + AAV9-GFP group. # P < 0.05 vs. TAC + AAV9-GFP group.

    Techniques Used: Western Blot, Expressing



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    Image Search Results


    Representative western blot and quantitative data of p-YAP1, YAP1, p-AKT, AKT, p-GSK3β and GSK3β protein expression (n = 4 per group). * P < 0.05 vs. CTL + AAV9-GFP group. # P < 0.05 vs. TAC + AAV9-GFP group.

    Journal: PLOS ONE

    Article Title: Upregulated LIMD1 alleviates pressure overload-induced cardiac hypertrophy via inhibits YAP1/AKT/GSK3β signaling

    doi: 10.1371/journal.pone.0316149

    Figure Lengend Snippet: Representative western blot and quantitative data of p-YAP1, YAP1, p-AKT, AKT, p-GSK3β and GSK3β protein expression (n = 4 per group). * P < 0.05 vs. CTL + AAV9-GFP group. # P < 0.05 vs. TAC + AAV9-GFP group.

    Article Snippet: Following blocking with 5% (w/v) non-fat dry milk, the membranes were incubated with primary antibodies against LIMD1 (1:500, Abclonal, A17585), phosphorylated YAP1 (1:500, CST, 13008), YAP1 (1:1000, CST, 14074), phosphorylated AKT (1:1000, CST, 9018), AKT (1:2000, CST, 9272), phosphorylated GSK3β (1:1000, CST, 9323), GSK3β (1:2000, CST, 9315), and GAPDH (1:10000, Abcam, Ab181602).

    Techniques: Western Blot, Expressing

    GALNT12 enhances malignancy by facilitating YAP1 nuclear localization. (A) Gene Set Enrichment Analysis was performed on the GSE2553 dataset. (B) Protein expression levels of GALNT12, YAP1, p-YAP1 and GAPDH were measured in transfected HT-1080 cells using western blotting. (C) Protein expression levels of YAP1 in the nucleus and cytoplasm of cells were measured and (D) the ratio of localization was quantified. One-way ANOVA (followed by Tukey's Honest Significant Difference test) was used to analyze the differences between the three groups. (E) Relative mRNA levels of AMOTL2, AREG, BIRC5, CTGF and CYR61 were detected using reverse transcription-quantitative PCR. siGALNT12-1 was used for this assay to interfere with the expression of GALNT12. Unpaired Student's t-tests were used to analyze the differences between the two groups. Data were presented as the mean ± standard deviation (n=3). *P<0.05; ****P<0.001. ns, not significant; si, small interfering; NC, negative control; GALNT12, polypeptide N-acetylgalactosaminyltransferase 12; YAP1, yes1 associated transcriptional regulator; p, phosphorylated; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; AREG, amphiregulin; AMOTL2, angiomotin-like 2; BIRC5, baculoviral IAP repeat containing 5; NES, normalized enrichment score; FDR, false discovery rate.

    Journal: Oncology Letters

    Article Title: GALNT12 promotes fibrosarcoma growth by accelerating YAP1 nuclear localization

    doi: 10.3892/ol.2023.14131

    Figure Lengend Snippet: GALNT12 enhances malignancy by facilitating YAP1 nuclear localization. (A) Gene Set Enrichment Analysis was performed on the GSE2553 dataset. (B) Protein expression levels of GALNT12, YAP1, p-YAP1 and GAPDH were measured in transfected HT-1080 cells using western blotting. (C) Protein expression levels of YAP1 in the nucleus and cytoplasm of cells were measured and (D) the ratio of localization was quantified. One-way ANOVA (followed by Tukey's Honest Significant Difference test) was used to analyze the differences between the three groups. (E) Relative mRNA levels of AMOTL2, AREG, BIRC5, CTGF and CYR61 were detected using reverse transcription-quantitative PCR. siGALNT12-1 was used for this assay to interfere with the expression of GALNT12. Unpaired Student's t-tests were used to analyze the differences between the two groups. Data were presented as the mean ± standard deviation (n=3). *P<0.05; ****P<0.001. ns, not significant; si, small interfering; NC, negative control; GALNT12, polypeptide N-acetylgalactosaminyltransferase 12; YAP1, yes1 associated transcriptional regulator; p, phosphorylated; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; AREG, amphiregulin; AMOTL2, angiomotin-like 2; BIRC5, baculoviral IAP repeat containing 5; NES, normalized enrichment score; FDR, false discovery rate.

    Article Snippet: The primary antibodies used were: GALNT12 (cat. no. K108365P; Beijing Solarbio Science & Technology Co., Ltd.), GAPDH (cat. no. GB15004; Wuhan Servicebio Technology Co., Ltd.), yes1 associated transcriptional regulator (YAP1; cat. no. ET1608-30; HUABIO), phosphorylated (p)-YAP1 (cat. no. ET1611-69; HUABIO) and Lamin B1 (cat. no. ET1606-27; HUABIO).

    Techniques: Expressing, Transfection, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation, Negative Control

    Schematic diagram of GALNT12 regulating YAP1 nuclear localization. YAP1, yes1 associated transcriptional regulator; p, phosphorylated; GALNT12, polypeptide N-acetylgalactosaminyltransferase 12; AMOTL2, angiomotin-like 2; BIRC5, baculoviral IAP repeat containing 5; CYR61, cysteine-rich angiogenic inducer 61.

    Journal: Oncology Letters

    Article Title: GALNT12 promotes fibrosarcoma growth by accelerating YAP1 nuclear localization

    doi: 10.3892/ol.2023.14131

    Figure Lengend Snippet: Schematic diagram of GALNT12 regulating YAP1 nuclear localization. YAP1, yes1 associated transcriptional regulator; p, phosphorylated; GALNT12, polypeptide N-acetylgalactosaminyltransferase 12; AMOTL2, angiomotin-like 2; BIRC5, baculoviral IAP repeat containing 5; CYR61, cysteine-rich angiogenic inducer 61.

    Article Snippet: The primary antibodies used were: GALNT12 (cat. no. K108365P; Beijing Solarbio Science & Technology Co., Ltd.), GAPDH (cat. no. GB15004; Wuhan Servicebio Technology Co., Ltd.), yes1 associated transcriptional regulator (YAP1; cat. no. ET1608-30; HUABIO), phosphorylated (p)-YAP1 (cat. no. ET1611-69; HUABIO) and Lamin B1 (cat. no. ET1606-27; HUABIO).

    Techniques: